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Fig. 4. (A–H) Expression of Psf2 during development. Embryos in (A), (F–H) are oriented anterior to the left and dorsal to the top; those in (C–E) which are dorsal views with the anterior to the top. (A) Stage 14 embryo. Arrowheads demarcate weaker regions of expression outside the neural plate, within the presumptive placodal regions. (B) Stage 22 embryo, anterior view with the dorsal side to the top. (C) Stage 22 embryo. (D) Stage 31 embryo. The bracket demarcates the right half of the CNS, and the left margin of the bracket aligns with the inner boundary of the ventricular zone. (E) Stage 37. (F) Stage 26. (G)
Stage 31 embryo. (H) Stage 35 embryo. (I) Transverse section of a stage 31 embryo, dorsal to the left, showing expression in the optic cup, lens, and ventricular zone of the CNS. (J–M) Expression of Psf2 during cornea-lens transdifferentiation. Arrowheads demarcate the region of stain in (J–L) (J) Relatively diffuse
Psf2 expression in the cornea overlying the pupillary space three days after lens-ectomy. This stain does not appear directly over the pupillary space due to the differential contractions of the eye muscles resultant from the fixation process. (K and L) Expression six days after lens-ectomy, localized within the newly forming lens vesicle. (M) Undisturbed, control eye showing no detectable expression of Psf2 in the cornea. (N) Sense control stage 23 embryo. (O) Sense control stage 31 embryo. (P) Sense control stage 37 embryo so: somites. All other labels follow that listed in the legends to Figs. 1–3. The scale bar in (M) equals 380 mm in (A and B), 760 mm in (C), 200 mm in (D and E), 550 mm in (F–H), (N–P), 240 mm in (I), and 400 mm in (J–M). |
