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Fig. 2. cfap206 is co-expressed with and dependent on foxj1 in Xenopus laevis. (A) Analysis of foxj1 and cfap206 mRNA expression in staged embryos using antisense RNA probes. (a,e) In gastrula stage 10 embryos, foxj1 transcripts were present in the LRO precursor, the superficial mesoderm (SM; a), while cfap206 was not detected by in situ hybridisation (e). Histological sections (b′,f′; planes of sections indicated by red dashed lines in b,f) of stage 19 dorsal explants (b,f) revealed overlapping expression of foxj1 (b,b′) and cfap206 (f,f′) in the floor plate (FP), while in the gastrocoel roof plate (area of LRO; outlined by dashed lines in b,f), only cfap206 transcripts were detected. Staining in b reflects expression in the floorplate above the LRO. (c,g) In stage 29 larvae, both genes were co-expressed in the nephrostomes (white arrowheads) and MCCs. (d,h) In the head of stage 45 tadpoles, strong neural expression of foxj1 was seen in the sub-commissural organ (SCO), zona limitans intrathalamica (ZLI) and FP (d‴). Transcripts of cfap206 were detected in the same tissues; however, at reduced levels (h‴). Non-neural expression was found in the stomach (stom.; d′,h′) and in dorsal cells lining the branchial chamber (BC; d′,h′). (B,C) cfap206 is a foxj1 target gene. (B) Strong cfap206 induction in embryos unilaterally injected with foxj1 mRNA. Asterisk indicates injected side. (C) Reduction of cfap206 expression in stage 24 foxj1 F0 crispant embryos (b,b′) when compared with wild type (a,a′). Boxed areas in a,b indicate the regions shown at higher magnification in a′,b′. Scale bars: 100 µm. |
