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Fig. 1. Pronephric expression of Xenopus Tbx2 analyzed by in situ hybridization. (A,B) Expression of Tbx2 around the pronephric anlage (pa) at stage 21 (A) and 23 (B). Arrowhead indicates weakly expressed Tbx2 around the pronephric anlage. (C) Transverse section of the embryo in B at the level indicated by the dashed line. (D) Tbx2 expression surrounding the developing pronephric tubule and duct at stage 31/32. (E) Magnified view of the boxed area in D. Arrowheads indicate the increased expression of Tbx2 in the dorsal region of the pronephric tubule anlage. (F,G) Transverse sections of the embryo in D at the levels indicated by the dashed lines. The arrow (F) indicates the absence of expression of Tbx2 in the medial layer of the intermediate mesoderm (IM). (H) Tbx2 expression enclosing the pronephric tubule (pt) and duct (pd) at stage 35/36. (I) Enlarged view of the boxed region in H. Arrowheads point to the expression of Tbx2 around the tips of nephrostomes. (J,K) Transverse sections of the embryo in H at the levels indicated by the dashed lines. The pronephric glomus (g) is outlined in red in J. The arrow indicates Tbx2 expression in the non-glomus mesenchyme in the medial layer of IM. (L) Pax2 expression in the pronephric anlage at stage 21. (M-O) Pax2 expression in transverse sections of the pronephric anlage, tubule and duct at the indicated stages. (P) Strong expression of Pax2 in the tips of nephrostomes (arrowheads). |