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Fig. 1. katnal2is expressed in ciliated tissues and the developing nervous system. A-H) Whole mount RNA in situ hybridization for katnal2 during X. tropicalis development. A) katnal2 expression is enriched in the dorsal mesoderm (dm). A’) Transverse section of A. B) Dorsal view of katnal2 expression in the floor plate (fp) and epidermis (epi). B’) Lateral view of B, anterior to the left also showing notochord (nc) staining. C) Lateral view of katnal2 expression enriched in the nc, pronephric nephrostomes (pn), and pharyngeal arches (pa). C’) Higher magnification view of C highlighting pn and nc expression. D) Lateral view of katnal2 expression in the spinal cord (sc) and otic vesicle (ov). D’) Dorsal view of katnal2 expression in the brain (br), especially in the cells lining the ventricles. E) Sagittal section of stage 15 embryo showing expression in the gastroceol roof plate (grp), anterior to the blastopore (bp). F) Coronal section showing an interior dorsal view of a stage 15 embryo, with expression in the nc and grp. G) Transverse section of a stage 30 embryo showing expression in the neural tube (nt), floor plate (fp), and notochord (nc). H) Coronal section of stage 38 embryo showing high expression in the cells lining the telencephalic (tel) ventricle (vent). Abbreviations: dm = dorsal mesoderm; fp = floor plate; epi = epidermis; nc = notochord; pn = pronephric nephrostomes; pa = pharyngeal arches; ov = otic vesicle; sc = spinal cord, br = brain; grp = gastroceol roof plate; bp = blastopore; nt = neural tube; vent = ventricle; tel = telencephalon. Scale: 100 μm, except H where Scale: 10 μm. |
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katnal2 (katanin p60 subunit A-like 2 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior left, dorsal up. |
