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Fig. 3. In silico screening for candidates with similar properties to SC genes. (A) Schematic of the in silico screen. Known SC disease genes served as an input list. Resource lists represent datasets from various published screening approaches and were tested for enrichment of genes contained in the input list genes. Genes in significantly enriched resource lists were then scored based on membership or rank. The gene scores were statistically validated by an empirical P value based on 10 million random drawings of input list. (B) Whole-mount in situ hybridizations for four candidate genes (c11orf74, cep192, cfap20, and ttc30a) that were experimentally followed up are shown for tadpoles at stage 31 to 35 (nephrostome expression enlarged) and limb buds (stage 55 to 57). (Scale bars, 0.5 mm.) p, posterior; a, anterior; pr, proximal; and d, distal. |
