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Fig. 1. Expression and localisation of Cfap157 RNA and protein. (A) RT-PCR with primers in exons 1 and 3 of Cfap157, in exons 2 and 3 of Foxj1, and in exons
7 and 9 of Hprt on RNA isolated from E18.5 and adult wild-type mouse tissues. Boxes indicate co-expression of Cfap157 and Foxj1 in tissues carrying motile cilia.
Asterisk indicates Cfap157 PCR product in kidney that was not consistently detected by RT-PCR (see Fig. 3D). (B) Section in situ hybridisation with a full-length
Cfap157 cDNA probe on E18.5 and adult wild-type mouse tissues, as well as E18.5 Foxj1 wild-type (wt) and Foxj1-deficient (Foxj1−/−) sagittal nose sections.
Arrows indicate expression domains. Scale bars: 500 µm in a-c,e,f; 250 µm in d. (C) Whole-mount in situ hybridisation of Xenopus laevis embryos with a full-length
cfap157 cDNA probe at stage 16 (a, dorsal explant; a′, transverse section along plane shown in a; arrow, gastrocoel roof plate), stage 35 (b; arrowhead,
nephrostomes; arrows, multiciliated epidermal cells) and stage 45 (c; transverse section of tadpole; s, stomach). Scale bars: 100 µm. (D) Whole-mount in situ
hybridization with a full-length cDNA foxj1 probe (a) or a cfap157 probe (b) on stage 20 Xenopus embryos after left-sided injection of foxj1 mRNA (n=37). Scale
bars: 100 µm. (E) Localisation of CFAP157-GFP protein at the basal body of IMCD3 cells co-stained with acetylated tubulin (a-c) or γ-tubulin (d-f ). Arrows,
CFAP157-GFP. Scale bars: 5 µm in a-c; 2 µm in d-f. (F) Localisation of CFAP157-GFP protein to basal bodies of motile cilia on cells of stage 30 Xenopus larval
epidermis co-stained with γ-tubulin. Scale bar: 1 µm. |
