| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| ddx59 | xenopus | germ plasm [+] |
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Experiment details for ddx59
Centroid, a novel putative DEAD-box RNA helicase maternal mRNA, is localized in the mitochondrial cloud in Xenopus laevis ooc...Centroid, a novel putative DEAD-box RNA helicase maternal mRNA, is localized in the mitochondrial cloud in Xenopus laevis oocytes.
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| Fig. 4. Electron microscopy analysis of centroid mRNA localization in the germ plasm of Xenopus oocytes. (A) Fragment of the mitochondrial cloud in a pre-stage I oocyte hybridized with a centroid anti-sense RNA probe, labeled with nanogold and silver-enhanced showing the mitochondrial cement (long arrows) located between the mitochondria. Centroid mRNA (short arrows) is present in the mitochondrial cloud but excluded from the mitochondrial cement. (B,C) Fragment of the mitochondrial cloud from stage 2 oocytes showing centroid mRNA (short arrows) located in the vicinity (B) and at the periphery (C) of the germinal granules (long arrows). m, mitochondria. | ||||||||||||||||
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| Fig. 3. Centroid mRNA localization in Xenopus oocytes. (A-C) Whole mount in situ hybridization showing localization of centroid mRNA (arrows) in the mitochondrial cloud in a stage 1 oocyte (A) and in the apex of the vegetal cortex in a stage II oocyte (B) and a stage III oocyte (C). (D- J) Sections of whole mount in situ hybridization showing localization of centroid mRNA in oocytes at different stages. (D) In a pre-stage I oocyte, centroid mRNA is uniformly dispersed in the cytoplasm but excluded from the mitochondrial cloud (long arrow) and secondary clouds (short arrows). (E,F) In a stage I oocyte, centroid mRNA (arrow) is located in the center of the mitochondrial cloud. Panel (F) shows the high magnification of the mitochondrial cloud (white sphere) with centrally located centroid mRNA (arrow). (G,H) In late stage I/early stage II and stage II oocytes, centroid mRNA is limited to the vegetal tip of the mitochondrial cloud (co- localizing with the germ plasm [long arrow]) and is excluded from the apical part of the mitochondrial cloud (short arrows). (I-K) Stage III, early stage IV and stage IV oocytes showing localization of centroid mRNA in the islands of a dispersing mitochondrial cloud (arrows). n, nucleus. Scale barsareequalto56μmin(A),90μmin(B,G),100μmin(C),70μmin(D), 75 μm in (E), 65 μm in (F), 86 μm in (H), 80 μm in (I) and 100 μm in (J,K). | ||||||||||||||||
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| ddx59 (DEAD-box helicase 59) gene expression in Xenopus laevis embryos, oocyte stage III, as assayed by in situ hybridization, transverse section, animal pole up. |