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FIG. 4. Whole-mount in situ hybridization of XFGF-8 to early limb bud stages. (A and B) Ventral and distal view, respectively, of a stage 50 limb bud. XFGF-8 is expressed along the dorsoventral boundary in a broad and fuzzy band. (C and D) Longitudinal sections through a stage 50 limb bud. Phase contrast (C) and bright field (D) picture of the same section. XFGF-8 transcripts are confined to ectodermal cells. Note that no morphological AER is visible at this stage. (E) Dorsal view of an early stage 52 limb bud. XFGF-8 transcripts are concentrated in the anterior ridge. (F) Ventral side of a tilted stage 56 limb. XFGF-8 expression confined to connective tissue on the ventral and dorsal side of the digits. |