| |
FIG. 4. Expression of the PCR-generated fragment in frog
melanophores (M) and fibroblasts (F). 1 pg of poly(A') RNA
was electrophoresed on 1.5% agarose/formaldehyde gel, transferred
onto nylon, and hybridized at 42 'C in 50% formamide solution for
12 h with a random-hexamer primer labeled PCR-generated fragment.
The blot was washed with 0.1 X SSPE with 0.1% SDS solution at
65 "C for 20 min and exposed to x-ray film with intensifying screen
at -80 'C for 10 h. The quality of the RNA in both lanes was
confirmed by staining the blot with 0.2% methylene blue and was
found to be same in both lanes. MW, RNA ladder in kb |
