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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
aqp3	xenopus		involuted dorsal mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior notochord,archenteron roof midline,axial ossicle,caudal presomitic mesoderm,cranial skeleton,dermatome,dermomyotome,dorsal endoderm,dorsomedial edge of myotome,flexural organ,left-right organizer,left-right organizer primordium,mandibular mesenchyme,monociliated cell,myocoelic slit,myotome,notochordal sheath,perichordal tissue,postcranial axial skeleton,posterior notochord,prechordal plate,presomitic mesoderm,rib,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,stratum compactum,stratum spongiosum,syndetome,transverse process,urostyle,vacuolated notochordal tissue,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,axial mesoderm,axial skeleton,cervical vertebra,dermatome,dermis,elastica externa,flexural organ,gastrocoel roof plate,head somite 1,head somite 2,head somite 3,head somite 4,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,notochord,notochordal epithelium,paraxial mesoderm,presomitic mesoderm,presumptive axial mesoderm,presumptive paraxial mesoderm,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,somite,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle

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Experiment details for aqp3

Roles for Xenopus aquaporin-3b (aqp3.L) during gastrulation: fibrillar fibronectin and tissue boundary establishment in the ...

Roles for Xenopus aquaporin-3b (aqp3.L) during gastrulation: Fibrillar fibronectin and tissue boundary establishment in the dorsal margin.

Gene	Clone	Species	Stages	Anatomy
aqp3.L		laevis	NF stage 11	involuted dorsal mesoderm
aqp3.L		laevis	NF stage 12	involuted dorsal mesoderm

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Fig. 1. aqp3bis expressed in the marginal zones of gastrula embryos. aqp3b expression was characterized by in situ hybridization in gastrula Xenopus embryos. (A-C) Animal pole views of aqp3b expression in stage 10.5 embryos. Expression of aqp3b was strong in the entire animal cap (A) and otx2 expression marks the dorsal lip of the blastopore (B). (C) Double in situ hybridization showed aqp3b (bracket) and otx2 (arrow) expression. The aqp3b expression domain extended to the region of otx2 expression. (D-I) Hemisected embryos. (D) aqp3b is expressed in the sensorial layer of the blastocoel roof and in the marginal zones at early gastrula stage 10. (F) aqp3b expression (bracket) extends to the dorsal lip of the blastopore (marked by otx2, arrow; stage 10.25 embryo). The strong staining lining the blastocoel was also present in otx2 single in situ hybridizations and therefore due to the otx2 probe (not shown). Stage 11 (G) and stage 12 (I) embryos showed diminishing levels of aqp3b expression in involuted mesoderm (arrowheads). In situ hybridization with aqp3b sense probe demonstrated low levels of background in the areas of interest (E, H). Remnants of brown pigment are due to the use of bleached wild type embryos. The dorsal lip of the blastopore is at the top in (A-C) and to the right in (D-I). D, dorsal; V, ventral.