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Fig. 4.
Whole mount in situ hybridisation analysis of Xenopus embryos. (A) Frontal view of stage 18 embryo showing that Xapy is enriched in the developing cement gland (cg), a patch of anterior epidermis just ventral to the neural tube (nt). (B) Lateral view of stage 24 embryo showing that Xapy continues to be expressed the cement gland. (C–D) Lateral view of a stage 30 (C) and a stage 40 (D) embryo showing Xapy expression in the head (h) and notochord (n). Xapy continues to be expressed in the cement gland, but is restricted to the basal region. In (B–D), the head of the embryo is on the right. (E–F) Sections through the head of a stage 20 (E) and a stage 30 (F) embryo, showing that expression is highly enriched in the cement gland. Note that in (F) staining is localized to the basal region of the cement gland. p = pharynx, b = brain. |
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cant1(calcium activated nucleotidase 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, laterial view, anterior right. |
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cant1(calcium activated nucleotidase 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, transverse section. |
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cant1(calcium activated nucleotidase 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 40, lateral view, anterior right. |
