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Fig. 2. Whole-mount immunolocalization of SPARC during early Xenopus embryogenesis. An anti-SPARC monocional anti-body(MAb-ON3), which
cross-reacted with Xenopus SPARC, was used to localize SPARC (A) During early somitogenesis (stage 18).SPARC was detected within the notochord
(n), the unsegmented dorsal mesoderm (urn), segmented somites (51,and eye anlage (ea), (8) By stage 25 the notochord tube (n), eyes (e) and somites
(s) all expressed high levels of SPARC. At higher magnification (E) SPARC was found within the samires. and was becoming detectable within the
intersomitic cleft (i). (C) By stage 33 SPARC levels decreased In the notochord (n), but remain elevated within the neural tube (nt). The protein exhibited
a striated chevron distribution along the somites. At higher magnification (F) this striation could be seen as a result of SPARC accumulation within the
intersomitic clefts (i). (D) The stage-45 embryo showed decreased levels of SPARC in the dorsal a1(;s, bur increased anterior accumulation. As a control.
a neural-specific antibody (G) was used. |
