| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| krt15.2 | xenopus | hindlimb bud [+] |
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Experiment details for krt15.2
Tazawa I et al. (2006) AssayA novel Xenopus laevis larval keratin gene, xlk2: its gene structure and expression during regeneration and metamorphosis of limb and tail.
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| Fig. 3. Spatial expression of xlk2 in developing hind limbs. (A) Whole mount in situ hybridization was performed on developing hind limbs of tadpoles at the indicated stages with the antisense probe. The figures represent views of the dorsal side of the limb. (B) A control experiment of A using the sense probe. (C) A section of the limb at the stage 55 shown in A. The arrow indicates the dorsoventral direction. The arrowheads point to the regions where xlk2 was not expressed. (D) Magnification of the region enclosed by the rectangle in C. (E) A serial section of D was stained with hematoxylin. The insets in D and E show magnified figures of the areas enclosed by rectangles. a, the apical layer; b, the basal layer; c, collagen layer; and m; mesenchyme. The magnification of B and E is identical to A and D, respectively. Scale bars = 1 mm in A, and 100 μm in C and D. | ||||||||||||||||
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| Fig. 5. Spatial expression of xlk2 during regeneration of hind limbs and tails. [I] Limb regeneration. Limbs of tadpoles at stage 54 were amputated and were subjected to whole mount in situ hybridization for xlk2 mRNA at 3 days post-amputation with the antisense (A) and the sense probe (B). Three limbs are shown. The closed arrowheads point to the amputation sites. The right limb in A is sectioned and shown in C. A serial section of C was stained with hematoxylin (D). The magnification of B and D is identical to that of A and C, respectively. Scale bars = 1 mm in A, and 200 μm in C. [II] Tail regeneration. Normal tails of tadpoles at stage 50 were subjected to whole mount in situ hybridization for xlk2 mRNA. A representative photograph is shown in A. Tails of tadpoles at stage 49–50 were amputated and subjected to whole mount in situ hybridization for xlk2 mRNA at 3 days post-amputation with the antisense (B) and the sense probes (C). Three tails are shown. There were non-specific signals in the notochord of tails and the mesenchyme of regenerates. The closed arrowheads point to the amputation sites and the open arrowhead in the left sample in C points to the notochord. The left tail in B is sectioned and shown in D. A serial section of D was stained with hematoxylin (E). Similarly, whole mount in situ hybridization was performed at 5 days post-amputation with the antisense (F) and the sense probes (G). The dotted line of the left sample in F indicates the regenerate. A longitudinal section was made from a proximal and distal region of the left tail shown in F and is shown in H and J, respectively. Serial sections of H and J were stained with hematoxylin and are shown in I and K, respectively. The magnification of B, C, F, and G is identical to that of A. The magnification of sections of D, E, and H–K is identical and its scale bar is shown in D. Scale bars = 1 mm in A, and 100 μm in D. |