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Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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grin2b | xenopus | hindlimb bud [+] |
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Experiment details for grin2b
Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus.
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Figure 5. Switch from glutamate to acetylcholine receptors in hindlimb muscles. A. Examples of hindlimb innervation patterns and distribution of ACh nicotinic receptors in a whole-mount hindlimb bud, revealed by fluorescence immunolabeling of neurofilament associated protein (Neurofil., red), glutamate receptor (NR2b, blue) and α-bungarotoxin labeling (α-bungaroTx, yellow) respectively, at stages 52 (upper panels) and 57 (lower panels). Inset drawings at bottom left of each panel show bud morphology at the two representative larval stages. Scale bars: 200μm for stage 52, 100μm for stage 57. B. Examples of fluorescence immunolabeling against neurofilament associated protein (red), synaptophysin (Synaptoph., green) and NMDA glutamate receptor subunit 1 (NR1, blue) in whole-mount limb bud at stage 52. White arrowheads indicate sites of apposition of all 3 markers. Scale bar: 20μm. C. Examples of fluorescence immunolabeling against neurofilament associated transporter 1 (VGluT1, yellow) and the glutamate receptor subunit NR2b (blue) in whole-mount limb at stage 52. Scale bar: 50μm. |