Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
chatxenopus hindlimb bud [+] 

Too many results?Too few results?

Experiment details for chat

Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus.

Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus.

Gene Clone Species Stages Anatomy
chat.L laevis NF stage 52 to NF stage 65 hindlimb bud

Display additional annotations [+]
  Figure 5 supplement 1. VGluT1 expression appendicular MNs. A. Fluorescence immunolabeling against ChAT (red) and VGluT1 (yellow) in limb MNs retrogradely labeled with Alexa Fluor dextran 647 (AD 647, cyan) at stage 52. Whereas ChAT is absent from retrogradely labeled MNs, VGluT1 is expressed throughout almost the entire ventral horn, including in limb MN somata. Bottom images are magnifications of the area (a) delineated by the dotted line square in the merged image at top right. Punctiform VGluT1 expression occurred around MN somata (indicating labeling of glutamatergic presynaptic terminals) and a more diffuse VGluT1 signal also appeared in MN cytoplasm surrounding cell nuclei. B. Fluorescence immunolabeling against synaptophysin (Synaptoph., green), NMDA glutamate receptor subunit 1 (NR1, blue), VGluT1 (red) and neurofilament (Neurofil., grey) in hindlimb muscle tissue at stage 52 (B1) and stage 55 (B2). VGluT1 expression colocalized with synaptophysin and postsynaptic glutamate receptors were detected at stage 52, but not at stage 54. The bottom images in B1 are magnifications of the area (a) indicated in the merged image at top right. Scale bars = 20μm.