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Fig. 3. Ventral and lateral domains of Xphox2a and Xash1 expression. Patterns of gene expression were characterized as indicated by whole-mount in situ hybridization at neural plate/fold (A–E, G–L), neurula (F) and early tailbud stages (M–O). The ventral side of the embryo is shown in (A,E–G,I). In (B,H,J), both dorsal and ventral domains of XPhox2a, N-tubulin and Xash1 expression are visible in a neural plate/fold stage embryo. In (F), Xnkx2.5 staining is blue and Xphox2a positive cells are magenta. A transverse section through the neural plate of embryos probed for Xphox2a(C,D) or Xash1(K,L) is shown at low (C,K) and high (D,L) magnifications. |
