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Fig. 2. Whole-mount in situ hybridization analysis of XHas1 (A,B,B′,E,F,F′) and XHas2 (C,D,D′,G,H,G′) at neurula and early tailbud stages. (A) At stage 13, XHas1 is present in non-neural ectoderm. (B) At stage 17, XHas1 appears in the neural crest cells (NCC), red arrow. (B′) A stage 17, dissected embryo shows the XHas1 expression in the cephalic NCC. (B′) XFoxD3 mRNA expression in the cephalic NCC at stage 17. (C) At stage 13, XHas2 is expressed in paraxial mesoderm. (D,D′) At stage 18, XHas2 expression is confined in the pre-somitic mesoderm as shown in whole and dissected embryo. (E) At stage 21, XHas1 is down-regulated in the ectoderm and in the NCC. (F,F′) XHas1 expression in stage 24 whole embryo and in the corresponding histological section. (G,H) XHas2 distribution in stage 21 and stage 24 embryos. Yellow arrowhead: heart primordium. Black arrowhead: pronephric anlage. Red arrowhead: trunk neural crest cells. (G′) Frontal view of stage 21 embryo showing XHas2 expression in the head region. The arrow indicates the expression in a region containing the pituitary anlage. The pituitary anlage is revealed by the expression of Xlim-3 reported in the inset. |
