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Fig. 2.
The subcellular localization of endogenous cyclin B2 and the effect of the overexpression of the cyclin B2 N-terminus. Xenopus immature oocyte (Oo) and matured egg (Egg) extracts (A), and in vitro-synthesized cyclins B1 and B2 proteins in a reticulocyte lysate (B) were immunoblotted with affinity-purified anti-B2δN antibodies. As a control, the same volume of a reticulocyte lysate to which no mRNA was added was used. Arrowheads represent endogenous cyclin B2. Sizes of protein markers (kDa) are indicated on the left side. Immature uninjected oocytes (C) or oocytes injected with B2DC (D) are stained with anti-B2δN antibodies. A higher magnified image of the perinuclear region of uninjected oocytes is also shown (E). The same perinuclear regions of immature oocytes injected with B2DC (F), B2D (G) or B1DC (H) are stained with anti-B2δN antibodies. GV represents germinal vesicle. All Figures are meridional sections, and their animal pole points toward the upside. Scale bars represent150 μm (A and B) and 50 μm (C–F).
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