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Fig. 4. The IFABP-TRDN-GFP transgene specifically inhibits TH direct-response genes in the epithelium, but not in the mesenchyme. Cross sections of duodenums from (A, B) wild-type NF54 tadpoles treated with either 0 nM T3; (C, D) 5 nM T3 for 48 h. Cross sections of duodenums from (E, F) sibling tadpoles transgenic for IFABP-TRDN-GFP treated with 5 nM T3 for 48 h. (A, C, E) Expression of the TH direct-response gene, bZip, mRNA in the epithelium. (B, D, F) Expression of the TH direct-response gene, stromelysin-3 (ST3), mRNA in the mesenchyme. mRNA localized by in situ hybridization (purple stain). Scale bar in F denotes 0.2 mm length. |
