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Gene/CloneSpeciesStageAnatomy ItemExperimenter
appl2xenopus duodenum 

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Experiment details for appl2

Appl1 is essential for the survival of Xenopus pancreas, duodenum, and stomach progenitor cells.

Appl1 is essential for the survival of Xenopus pancreas, duodenum, and stomach progenitor cells.

Gene Clone Species Stages Anatomy
appl2.L laevis NF stage 41 to NF stage 43 duodenum

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  Supp. Fig. S2. Spatial and temporal expression of appl2 in Xenopus embryos. A–I: Spatial expression of appl2 in Xenopus embryos revealed by whole mount in situ hybridization. A: Lateral view of a blastula embryo with signals mainly detected in the animal hemisphere. B: Bisected half of a gastrula embryo showing weak and ubiquitous expression. C: Anterodorsal view. D: Anterior view. E: Lateral view. F: Dorsal view. G–I: Dissected guts probed with appl2. In comparison to those observed for appl1 (Fig. 1G–I) and akt2 (Fig. 5A), only faint signals in the pancreas and SD were detected after a much longer period of color reaction incubation during the last step of whole mount in situ hybridization. J: RT-PCR analysis reveals the temporal expression of appl2. UE, unfertilized egg; N, negative control. ODC was used as the RNA loading control.