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Figure 2: There is no detectable dorsal-ventral difference in mRNA localization of four neural transcription factors at cleavage stages. In situ hybridization detects the indicated mRNAs at the same relative intensities
in the dorsal (top) and ventral (bottom) 16- to 32-cell animal blastomeres, indicating a lack of localization.
Not shown: 1) there is no signal in vegetal hemisphere blastomeres; 2) similar results were observed when animal cap fragments were processed for ISH to ensure probe penetrance in the yolk-ladened cells; and 3) sense probes did not shown signal in either animal or vegetal hemispheres. |
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gmnn (geminin, DNA replication inhibitor) gene expression in Xenopus laeivs embryo, NF stage 6 (32-cell), lateral view, dorsal up, ventral down. |
