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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
emx1
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
eye
,
cranial neural crest
|
sox2
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
lens
,
brain
,
notochord
,
eye
,
[+]
|
pax6
|
|
laevis
|
NF stage 35 and 36
|
lens
,
forebrain
,
eye
|
nkx2-1
|
|
laevis
|
NF stage 35 and 36
|
floor plate
,
neural tube
|
en2
|
|
laevis
|
NF stage 35 and 36
|
hindbrain
,
heart
,
eye
,
endocardial tube
|
|
|
Fig 3. The CTLH complex functions during early embryonic neurogenesis.
(A) rmnd5-mo injected embryos were used for in situ hybridisation with indicated marker probes; pax6 (upper lane), n-tubulin (middle lane), k20/en22/rx1/c-actin, emx1.2, nkx2.1 (bottom lanes). Abbreviations: IS, injected side; NIS, non-injected side. Quantitative representation of phenotypes are presented as a bar graph (percent embryos with phenotype to total amount (%); black, phenotype; grey, no phenotype); n = number of independent experiments, N = number of injected embryos analysed for respective marker, *P ≤0.05, ***≤0.001 (Chi Square test). (B) As (A) with sox2 as probe. (C) Xenopus embryos co-injected with rmnd5 morpholino (2.5 pmol/embryo) and synthetic capped RNA (100 pg/embryo) were used for in situ hybridisation and quantified as shown in A. |
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Display additional annotations [+]
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S2 Fig. The CTLH complex functions during early embryonic neurogenesis.
As Fig. 3 with standard morpholino injected embryos |