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Figure 6. The role of HYLS-1 in ciliogenesis is conserved in vertebrates. (A) Schematic of Xenopus experiments. Inset shows ciliated epithelial cells stained for XHYLS-1, cilia (acetylated tubulin), and apical cell junctions (ZO-1). Immunoblot of control and XHYLS-1 morpholino (MO) tailbud-stage embryo extracts. (B) Basal bodies (γ-tubulin; green) are present, but cilia (acetylated tubulin; red) fail to assemble in XHYLS-1-depleted embryos. (C) Expression of wild-type but not disease mutant (D249G) XHYLS-1 fused to GFP restores ciliogenesis in XHYLS-1-depleted embryos. (D) Quantification of morpholino experiments. n = 10–12 embryos, 30 fields of view per embryo (cells with multiple cilia/basal bodies); 20 cells (basal bodies per cell). Bars, 10 μm. Error bars in D are the 95% confidence interval. |
