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Figure 4 - Dvl localizes near the base of cilia in multi-ciliated cells. (a–c) Dvl2 immunostaining shows enrichment at cell membranes throughout the epidermis and enrichment at the apical surface of ciliated cells, as indicated by immunostaining for centrin (b,c). Scale bar, 15 mum. (d) Signal for Dvl2 immunostaining (red) localizes immediately adjacent to the basal body, as indicated by Centrin2-GFP (green). Scale bar, 1 mum. (e) Signal for Dvl2 immunostaining (red) localizes to the center of the region marked by CLAMP-GFP (green); data in Supplementary Figure 4 indicate that CLAMP-GFP labels the ciliary rootlet. Scale bar, 1 mum. (f) The C terminus of Dvl2 is sufficient to drive localization to basal body in multi-ciliated cells. Stage 27 embryos are shown throughout. Scale bar, 5 um.
Image redisplayed with permission from Macmillan Publishers Ltd. |
