| |
Fig. 7. Cep4l and Fgf8a expand neurons and inhibit non-neuronal deep cell fates. ((A)–(E)) Expression of neuronal tubb2b (A), neural plate sox2 (B), neural crest sox9 (C), and superficial epidermal xk81a1 ((D) and (E) in embryos injected with 200 pg β-gal and 250 pg cep4l. ((F)–(I)) Cep4l misexpression in animal caps. Tubb2b expression in controls ((F) and (G)) or cep4l-injected ((H) and (I)) embryos and explants. ((K)–(P)) Expression of epidermal deep layer multiciliated cell marker crcc ((K)–(M)) and ionocyte marker foxi1 ((N)–(P)) in controls ((K) and (N)), cep4l-injected ((L) and (O)), and fgf8a-injected embryos ((M) and (P)). Insets in (N)–(P) show expression of tubb2b. |
