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rfx6xenopus stomach [+] 

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Experiment details for rfx6

Pearl EJ et al. (2011) Assay

Functional analysis of Rfx6 and mutant variants associated with neonatal diabetes.

Gene Clone Species Stages Anatomy
rfx6.L laevis NF stage 42 stomach

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  Fig. 1. Rfx6 expression in the endoderm and isolated gut tissue. (A) Sagittal bisection of an NF15 embryo showing an anteriorly localized stripe of rfx6 expression. (B) Sagittal bisection of an NF20 embryo showing rfx6 expression. (C) Transverse section through rfx6 expressing region of NF24 embryo showing broad expression in anterior endoderm. (D) NF28 embryo showing rfx6 expression restricted to a dorsal (arrow) and ventral (arrowhead) patch in the anterior foregut. (E) NF40 liver and pancreas showing rfx6 expression in the dorsal pancreas. (F) NF42 liver and pancreas showing rfx6 expression spreading to the ventral pancreas. (G) NF46 liver and pancreas showing rfx6 evenly expressed throughout the pancreas. (H) NF42 whole gut showing rfx6 expression in the dorsal pancreas, stomach and intestine. (A, B, D) Anterior is left. (A–D) Dorsal is up. (H) li, liver; p, pancreas; st, stomach. (I) RT-PCR of rfx6 in whole tadpoles at various stages throughout development.

Gene Clone Species Stages Anatomy
rfx6.L laevis NF stage 42 stomach

  rfx6(regulatory factor X, 6) gene expression in Xenopus laevis embryos, NF stage 42, as assayed by in situ hybridization. Lateral view of dissected foregut: anterior left, dorsal up.