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Figure 2. Spatial expression pattern of xPitx3 assayed by whole-mount in situ hybridization. A-C: Early stage embryos seen laterally from a slightly anterior perspective (right) in (A), head on (B), and from the right side with the head twisted to face out on the right (C). D: Anterior top view of a stage 30 embryo showing that xPitx3 is highly expressed in the differentiating lens and Rathke's pouch (the cement gland is at the right). E,F: A lateral view (E) and a ventral view (F) of a tadpole at stage 39. xPitx3 is expressed throughout heart and gut development. G-I: Sense control embryos (G) reveal no hybridization of probe, whereas both gut and heart display asymmetrical labeling during looping of both organs (H,I). BR, branchial arch; EA, eye anlage; J, jaw musculature; L, lens; OV, otic vesicle; R, Rathke's pouch; S, somite; S/H, stomodeal-hypophyseal anlage; h, heart. |
