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Gene |
Clone |
Species |
Stages |
Anatomy |
pou4f4
|
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laevis
|
NF stage 16
|
trigeminal placode
,
chordal neural plate border
|
pou4f4
|
|
laevis
|
NF stage 27
|
retina
,
cranial placode
,
lateral line placode
,
trigeminal nerve
|
pou4f4
|
|
laevis
|
NF stage 29 and 30
|
retina
,
brain
,
midbrain
,
cranial placode
,
cranial nerve
,
[+]
|
pou4f4
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
spinal cord
,
eye
,
neural tube
|
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FIG. 2. Developmental expression pattern of XBrn3d. Whole-mount in situ hybridization was performed on Xenopus embryos using a
DIG-labeled XBrn3d probe. (A) Expression commences at neurula stages in the trigeminal placodes (arrowhead) and a stripe of primary
neurons along the midline. (B) At stage 26–27, XBrn3d is first detected in the neural retina (arrow). Expression is also seen in the olfactory
placode (right of the retina) and cranial ganglia (left of the retina). (C) At stage 29, XBrn3d is expressed in the optic tectum (arrowhead) and
the spinal cord. (D) At stage 40, cross sections in paraffin-embedded embryos reveal strong retinal staining restricted to the retinal ganglion
cell layer and ciliary marginal zone (arrow) as well as the entire dorsal half of the neural tube at the level of the optic tectum (top, middle).
The pigment epithelial layer surrounds the neural retina (arrowhead) and is not labeled. (E, F) At stage 35, staining is strong in a region of
the developing optic vesicle and dorsal neural tube at the level of the hindbrain and spinal cord, respectively. |
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FIG. 3. Expression of XBrn3d and Xath5 overlaps in the ciliary marginal zone of the Xenopus retina. Double in situ hybridization was perform ed on stage 41 em bryos using an antisense DIG probe (XBrn3d) and an antisense fluorescein probe (Xath5). (A) Schem atic showing a cross section of the Xenopus retina. The retinal pigment epithelium is shown as a thick line. The retinal ganglion cell layer (RGC) is closest to the lens (L), while the photoreceptor layer (ON L) borders the pigm ent epithelium and the inner nuclear layer (IN L) is between. The ciliary marginal zone (CMZ) is the region of continued proliferation, in which the central CMZ is the portion closest to the differentiated retinal layers. (B) XBrn3d expression is restricted to the retinal ganglion cell layer and the central CMZ. (C) The composite im age of both the Xath5 and the XBrn3d staining shows double-labeled cells. (D) Xath5 is expressed in the CMZ. Double-labeled cells are indicated with brackets (B, D). |