| |
Fig. 3. Distribution of Xenopus prolactin receptor (PRL-R)
messenger ribonucleic acid (mRNA) in adult tissues. Total RNA
(each 2 μg) from various tissues of the juvenile frogs (6–12 months
after metamorphosis) was loaded on 1.2% agarose-formaldehyde
gel. The gel was stained with ethidium bromide (lower
panel) and used for detecting PRL-R mRNA by northern blot
hybridization (upper panel). Asterisks show the positions of ribosomal
RNA (28S and 18S rRNA). The main hybridization signal
was detected at approximately 8 kb in length (arrow). A faint
signal was also seen at approximately 3 kb (arrowhead) in
some tissues. In the skin RNA, no 8 kb signal was detected but
extra signals different from those described earlier were
observed. |
