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Fig. 1. Shroom3 is essential for morphogenesis of the developing Xenopus gut. (A) The expression pattern of Shroom3 in a normal stage 43 dissected gut. Shroom3 is robustly expressed in the foregut (arrow). (B,B′) Normal gut looping in a control stage 46 embryo injected with mRNA encoding GFP only (green in B′). (C,C′) Gut looping is disrupted following dominant-negative (DN)-Shroom3 expression (as marked by GFP, green in C′). (D,E) Control gut (D) and DN-Shroom3-injected (E) gut epithelium. GFP-positive gut epithelial cells display a high-columnar morphology in controls, whereas cells expressing GFP and DN-Shroom3 display more rounded shapes. |
