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lepxenopus hindgut [+] 

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Experiment details for lep

Immunocytochemical detection of leptin in non-mammalian vertebrate stomach.



Gene Clone Species Stages Anatomy
lep.L laevis NF stage 66 to adult frog stage enteric neuron

  Fig. 1. Scattered cells displaying the typical features of endocrine cells (arrows) are immunoreactive for leptin in the oxyntic glands of O. mykiss. Bar¼20 lm. Fig. 2. Pair of consecutive sections of the trout stomach immunostained for leptin. (A) The immunostaining appears in the enteric nervous system. (B) No immunoreactivity is seen after absorption of the antiserum with 20 lgml 1 of the leptin fragment [137–156]. Bar¼40 lm. Fig. 3. Panoramic view of the Xenopus oxyntic epithelium immunostained for leptin. The immunoreactivity appears specifically in the oxyntic-peptic cells. Bar¼40 lm. Fig. 4. Leptin-immunoreactive fibers in the myenteric plexus of Xenopus. B ar¼20 lm. Fig. 5. Immunocytochemical detection of leptin in the stomach of the snake. Positive cells are seen in the oxyntic glands and a very strong immunostain is also found in the enteric nervous system. Bar¼20 lm. Fig. 6. Two serial sections of the snake stomach showing colocalization of leptin (A) and VIP (B) immunoreactivity in the enteric nervous system. Bar¼40 lm.