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hspa9xenopus hindgut [+] 

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Experiment details for hspa9

Hspa9 is required for pronephros specification and formation in Xenopus laevis.

Hspa9 is required for pronephros specification and formation in Xenopus laevis.

Gene Clone Species Stages Anatomy
hspa9.L laevis NF stage 24 hindgut

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  Figure 1. Expression pattern of Hspa9 in Xenopus laevis embryo. A: RT-PCR hspa9 expression in Xenopus laevis embryos from stages 3 to 40 of development with odc1 as house-keeping gene. B–H: Whole-mount ISH of hspa9. B: Early gastrula, arrow indicates the position of the blastoporal lip. C: Early neurula stage (anterior view, dorsal side up) shows hspa9 expression in neural and mesodermal territories. D,E: hspa9 is expressed in somite (s), mesoderm (m) and intermediate mesoderm (im). F: hspa9 labels the pronephros anlage (pa). G: At early tail bud stage, strong expression of hspa9 in eye (e), otic vesicle (ov), branchial arches (ba), somites and pronephros, from proximal (red arrow) to distal part (black arrow). G′: Section through the plan of dotted line in G. H,H′: Tadpole stage shows persistent hspa9 expression in the pronephros tubule. I–L: hspa9 pronephros expression was compared with pax8 and pax2 renal markers. Arrows show similar hspa9 and pax expression domains, up to the tip of the nephric duct. A strong hspa9 expression is present in the proctodeum (asterisk in I, K). Abbreviations: dt, distal tubule; g, glomus; it, intermediate tubule; n, notochord; pt, proximal tubule. Scale bars = 0.5 mm in B–H; 1 mm in I–L.Download figure to PowerPoint