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Fig. 2. Temporal and spatial expression pattern of BENI during Xenopus development. (A) Temporal expression pattern of BENI during Xenopus development detected by RT-PCR. RT-PCR using an ornithine decarboxylase (ODC)-specific primer was carried out in parallel to control the amount of input RNA [ODC]. RT-PCR without reverse transcriptase showed no contamination of genomic DNA [ODC(RT−)]. (B–Q) Spatial expression pattern of BENI during development detected by whole-mount in situ hybridization. Embryos were hybridized with antisense (C, D, F, G, I, K, M–P, Q) and sense probes (B, E, H, J, L). (B, C) Stage 11, animal view. (D) Stage 11, vegetal view. (E, F) Stage 17, lateral view. (G) Stage 17, anterior view. (H, I) Stage 22, lateral view. (J, K) Stage 29/30, lateral view. (L, M) Stage 40, lateral view. (N–S) Transverse section of the embryo at stage 10.5. Panels P and Q are magnified views of panels N and O, respectively. Xbra expression (R–S). Scale bars, 100 μm. |
