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Fig. 5.
Developmental expression of Xenopuscalcineurin transcripts is visualized by in situhybridization of whole-mount albino Xenopus embryos with an xCN-specific antisense probe. A, xCN mRNA is not detected at the neural plate stage (stage 15). B,Message is first observed at the neural tube stage in the presumptive brain (stage 22). C, Transcripts are upregulated in the brain and spinal cord by the early tailbud stage (stage 26).D, Expression increases posteriorly as development progresses to the late tailbud stage (stage 32). E, A control tailbud stage embryo hybridized with xCN-specific sense probe does not reveal staining, indicating specificity of the antisense. For all embryos anterior is to the left, and dorsal isup. Scale bar, 500 μm. |
