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FIGURE 3. Comparative spatial expression profile of p2rx genes during embryogenesis. Whole-mount in situ hybridization with specific DIG-labeled antisense RNA probes was performed on embryos from stages (St.) 5 to 37/38. Representative embryos were photographed at the magnification X20 for stages 5, 11, and 14 and X16 for later stages. Stage 5: animal pole view, stage 11: vegetal pole view, stage 14: dorsal view except for p2rx1.S (anterior view), later stages: lateral views, with dorsal is up and anterior is right. The arrowheads indicate the staining in the neurogenic placodes and peripheral nervous system detailed in Figure 5. a.p, animal pole; c.g, cement gland; d.c, duct of Cuvier; e, eye; h, heart; h.b, hindbrain; k, kidney; m, mesoderm; n, notochord; n.s, nervous system; p.e.f, presumptive eye field; p.g, pineal gland; s, somite; t.n, trigeminal nerve; v.b.i, ventral blood island. |
