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Fig. S1. Endogenous expression of KCNQ1 mRNA. In situ hybridization with an antisense probe to native Xenopus KCNQ1 was performed on embryos at various
stages. (D–F) Embryos were embedded in JB4 medium and sectioned at 30 m after hybridization in whole mount. During neurulation, anterior neural plate
cells exhibited a strong signal, and expression also was detected in the neural folds (A) and in the mandibular, hyoid, and branchial neural crest streams (B). At
tailbud stage, embryos exhibited strong expression in the tail, branchial arches, face, and dorsal neural tube (C); stain in tailbud (D), neural tube (E), and
underlying cells around the cement gland (F) was confirmed in section. The extending tail maintained a focus of expression in under the spinal cord (G). Red
arrows indicate positive signal. cg indicates cement gland. |