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Figure S4 Expression pattern of XFLRT2 mRNA during Xenopus laevis
development. a-e, Whole-mount in situ hybridization of XFLRT2. Expression
is first seen anteriorly at early neurula stages (a, stage 14, anterior view). b,
Stage 18, animal view. c, Stage 24, lateral view. XFLRT2 is predominantly
expressed in the head. At tailbud stages, stage 32 (d) and stage 36 (e),
XFLRT2 transcripts are detected in the head and in the somites. f, RT-PCR
analysis of XFLRT3 and XFLRT2 expression in embryos of the indicated
stages. -RT, minus reverse transcription control sample; H4, histone H4 for
normalization. g, Sequence of the FLRT2 morpholino target region. An
alignment of Xenopus laevis (X.l. FLRT2) and tropicalis (X. trop. FLRT2)
FLRT2 nucleotide sequences is shown. A XFLRT2 pseudoallel was also
identified (not shown) whose sequence is identical in the XF2(b)-MO target
region but has two base-pair mismatches in the XF2(a)-MO target region. |
