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Fig. 4. Expression of Xevl transcripts during Xenopus development. Whole-mount in situ hybridization was carried out using a digoxigenin-labeled riboprobe that detects Xevl, Xevl-I and Xevl-H (Xevl−3′ probe; A–G) or Xevl-H (H–J). Nieuwkoop-Faber stages are indicated in lower right corners. Total Xevl expression in (A) stage 20, (B) stage 25, (C) stage 30, and (D) stage 35 embryos. (E-G) Transverse sections of stage 35 embryos at the level of the (E) eyes, (F) anterior neural tube, and (G) posterior neural tube. Xevl-H expression in (H) stage 20, (I) stage 30, and (J) stage 35 embryos. br, brain; cg, cement gland; ey, eye; LL, lateral line placode; myo, myotome; nt, neural tube; ol, olfactory placode; ot, otic vesicle. |
