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Figure 3. Expression of XDel1 RNA. A: Reverse transcriptase (RT) -polymerase chain reaction of XDel1 (upper row) and XHis4 (lower row) expression. Representative stages are shown: Roman numerals are for oocyte stages, and Arabic numerals are for embryo stages. B: Whole-mount in situ hybridization to XDel1. Gastrula embryo (stage 10.5), vegetal (left) and animal (right) views are shown. C: Mid-neurula embryo (stage 16), dorsal view (posterior to anterior, left to right). D: Tail bud embryo (stage 30), lateral view (posterior to anterior, left to right). E: Tail bud embryo (stage 25); close up of the head, XDel1 expression is enriched in the rostral rim of the cement gland (arrow). |
