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Fig. 6. (a) Expression of the An4 transcripts in Xenopus stage 26
embryo. After whole-mount in situ with a digoxigenin labelled An4a
cRNA probe, this stage 26 embryo was cleared. Staining of the forming
brain (arrows) including the optic cups (0) is seen through the
cleared head. (b) Expression of the An4 transcripts in Xenopus stage
33 embryo. Uncleared whole-mount in situ shows staining in brain (b)
and branchial arches (ba), but not in cement gland (c) or spinal cord
(8). (c) Expression of pTrCP transcripts in the Xenopus stage 25 embryo.
Uncleared whole-mount in situ of a stage 25 embryo with a
digoxigenin labelled antisense pTrCP-3 cRNA probe shows staining
in cement gland (c) of the head. |