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Fig.2. zfp36 mRNA is expressed in two different cell populations in early X. tropicalis embryos. Double sequential in situ hybridization (A) revealed for zfp36 and zfp36 + mpo in stage 18 and stage 26 embryos. Vibratome section of stage 20 embryos (B) stained for zfp36 or mpo as indicated. The cut plane is shown on the right panel. (C) Mixed view of stages 19-21 embryos stained for zfp36 or mpo. Arrowhead, anterior neural fold staining for zfp36, open arrowhead, posterior labeling of neural fold by zfp36. |