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Fig. 5. Ectopic Fusicoccin abolishes the asymmetry of 14-3-3E protein localization. (A,B) Control embryos exhibit 14-3-3E protein localization in only one blastomere. (C,D) By contrast, exposure to Fusicoccin abolishes the asymmetry and results in localization in both blastomeres. Sections in panels A and C were taken perpendicular to the animal-vegetal (AV) axis. Sections in panels B and D were taken parallel to the AV axis. Red arrowheads indicate localization; yellow arrowheads indicate lack of signal. Green line in panel B indicates cleavage plane between the blastomeres. |