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Fig. 2. Detection of specific mRNAs by whole-mount in situ hybridization. (A) Detection of muscle actin at different stages of Xenopus development. Embryonic stages 13 (dorsal view), 24 and 36 (lateral views) are presented from top to bottom. The blastopore of the stage 13 embryo is arrowed (bp). In addition to somites the heart (h) of the stage 36 embryo is stained. (B) Localization of the globin mRNA in the blood islands of a stage 32 tadpole. The staining is detected on the ventral side of the embryo and is bilaterally symmetrical along the anteroposterior axis. (C) Distribution of the XAG-1 transcript in the cement gland of a stage 23 embryo. Note that under saturating conditions of staining some non-specific staining is also detected on the dorsal side of the embryo. (D-F) Localization of the XA-1 transcript. (D) and
(E) represent two different views of a stage 23 embryo; (D) lateral view, (E) anterior view. (F) A stage 36 tadpole viewed in the same plane and magnification as (E). All embryos presented in this figure are albinos with the exception of (B) where a pigmented embryo was used. (A,B and C) have been cleared in benzyl-benzoate/benzyl alcohol to see deep staining; (D,E and F) have not been cleared. |
