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Fig. 5. Effect of Mix.1 and Mix.2 loss of functions upon later development of the pronephros. In situ hybridization analyses of XPax-2 (A–D) and XWT1 (E–H) in Mo1 or Mo3 injected embryos. Morpholinos were targeted on the right side as described in Fig. 4. On control sides, XPax-2 is expressed in nephrostome, tubules and Wolffian duct. Its expression is totally abolished in response to Mo1 or Mo3. Neural expression of XPax-2 in the hindbrain, optic and otic vesicles is also affected on the injected side. XWT1 is expressed in the glomus part of the pronephros on control sides, while it is totally down-regulated on the injected side both with Mo1 and Mo3. (I) Histological analysis at early tadpole stage (NF st. 38) after injection of Mo3 on the right side. Pronephros tubules differentiated on the control side (pt), are absent on the injected side. They are replaced by a mass of uncharacterized tissue (arrow). n: notochord; nt: neural tube; s: somites. |
