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Figure 7. Expression analysis of transcription factors. A
Whole-mount in situ hybridization analysis of Control and golph2-MO-injected embryos at stage 35; orientation: panels A, lateral view, the anterior towards the right; panels AL lateral view, the anterior towards the left. Morpholino (32 ng) was injected into a single ventral blastomere of 4-cell-stage embryos from the lateral region. (A WT1. Brackets indicate the expression domains. (C, Pax2; (E, Lim1; (G, Pax8; (I GATA3; (K, HNF1β. The arrow heads indicate reduced convolution of the pronephric tubules. |
