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Fig. 3. wt1 regulates podocyte gene expression. (A-I′) High magnification of representative transverse sections of whole-mount in situ hybridizations comparing the expression of podocyte genes wt1 (A,A′), tcf21 (B,B′), lmx1b (C,C′), mafb (D,D′), foxc2 (E,E′), ptpru (F,F′), nphs1 (G,G′), kirrel (H,H′) and nphs2 (I,I′) between wt1 morphants and sibling control Xenopus embryos at stage 35. (J) Wire diagram summarizing the in situ hybridization data. Arrows, positive regulation; T-bars, negative regulation; thick lines, strong effects; thin lines, weak effects. Note that none of the interactions is necessarily direct and might involve intermediary players. (K-L) In situ hybridization of nphs1 in uninjected controls (K), embryos injected with wt1-MO1 (K′) and with wt1* mRNA and wt1-MO1 (K′). The results of multiple experiments were quantified (L). Black, strong bilateral expression; white, loss of expression; gray, unilateral expression rescued by wt1* mRNA. |
