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Fig. 2. Knockdown of wt1 results in a glomus phenotype. (A) Sequences of the two pseudo-alleles of Xenopus wt1 and the location of wt1-MO1 and wt1-MO2. Mismatches between wt1-MO and the second allele of wt1 are indicated in red. The translational start site is boxed. (B) Schematic of the wt1-GFP reporter constructs. (C-E) Fluorescence of uninjected control embryos (C,D), embryos injected with the wt1-GFP reporter mRNA (C′), with wt1-MO1+2 and the wt1-GFP reporter (C′), wt1(mut)-GFP (D′) or with wt1-MO1+2 and the wt1(mut)-GFP (D′) at stage 10. The results of multiple experiments were quantified (E); the number of embryos analyzed is indicated above the bars. (F-K) Phenotype, histology and β1-Integrin immunofluorescence of wt1-MO1+2-injected embryos (G,I,K) and sibling controls (F,H,J) at stage 40 (F,G) and stage 42 (H-K). Red arrowheads indicate glomus in H. |
