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wnt8axenopus   

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Experiment details for wnt8a

Yao J and Kessler DS (2001) Assay



Gene Clone Species Stages Anatomy
wnt8a xenopus unspecified stage

 

Gene Clone Species Stages Anatomy
wnt8a.L laevis NF stage 10.25 ventro-lateral marginal zone

  Fig. 5. Cell autonomous activation of Xwnt8 by VP16-Gsc. At the 16-cell stage, one dorsal marginal zone blastomere was injected with 750 pg of β-galactosidase (β-gal) mRNA (A) or a combination of β-gal and 500 pg of VP16-Gsc (B). Descendents of the injected cell were identified at the gastrula stage by the presence of β-gal, using the Rose-gal substrate. Activation of Xwnt8 by VP16-Gsc in the dorsal marginal zone and endogenous ventrolateral expression of Xwnt8 was detected by in situ hybridization with BM-purple substrate (A,B). Dorsal views of stage 10.25 embryos with endogenous Xwnt8 expression visible in lateral regions. Insets show higher magnification views of boxed regions. Black arrowheads indicate β-gal-positive nuclei (red) and the white arrowhead indicates cytoplasmic Xwnt8 in situ stain (purple). Scale bar: 0.25 mm; 80 μm in the insets.

Gene Clone Species Stages Anatomy
wnt8a.L laevis NF stage 10.5 mesoderm , marginal zone , ventro-lateral marginal zone

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  Fig. 8. VP16-Gsc alters gene expression at the gastrula and neurula stages. At the four-cell stage, two dorsal blastomeres were injected with 500 pg of VP16-Gsc. Embryos were fixed at stage 10.5 (early gastrula), 12.5 (late gastrula), 15 (mid-neurula) or 20 (late neurula). Gene expression was detected by in situ hybridization of uninjected (Control) and VP16-Gsc-injected embryos. VP16-Gsc caused an expansion of Xwnt8 (B) and MyoD (F) into the dorsal marginal zone at stage 10.5, and MyoD-positive cells were present at the midline (white arrow) at stage 15 (H). Frzb and Chordin expression in the organizer was not affected at stage 10.5 (D,J) and Chordin expression in the chordamesoderm was normal at stage 15 (L). Otx2 was reduced or absent at all stages examined (N,P,R,T). Opl expression in the prospective neural plate was not affected by VP16-Gsc at stage 10.5 (V), but was absent from the most anterior domain of the neural plate (bracket) at stage 12.5 (X). Black arrowheads indicate the dorsal blastopore lip. The black arrows indicate the primordia of the forebrain (fb), eyes (e) and cement gland (cg) (S). Vegetal views are shown in A-F,I,J,M,N, dorsal view is shown in G,H,K,L,U,V with anterior on the left in G,H,K,L and anterior view is shown in O-T,W,X. Scale bar: 0.5 mm.