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Figure S4. Regulation of wnt8a transcription by Pnhd. A-D, Whole mount in situ hybridization validates changes in wnt8a expression in embryos with manipulated pnhd. Injection experiments were performed as described in Fig. 5B- F. Wnt8 transcripts are visible as dark blue staining. RedGal is a lineage tracer. Representative embryos are shown. E, quantification of the results shown in A-D. F, RT-qPCR confirms downregulation of wnt8a.L, tbxt.s, msgn1.L and hoxd1.s in stage 10.5 marginal zone explants depleted of Pnhd. By contrast, the ventral marker admp2 is not affected by Pnhd depletion. |
