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Fig. 4. Expression of mesoderm, endoderm and neural markers in Tbx6VP16-injected early gastrula. Embryos at 4-cell stage were dorsally injected with Tbx6VP16 mRNA and cultured to early gastrula stage for in situ hybridization. (A) Vegetal view of an uninjected early gastrula. (B) Dorso-vegetal view of a Tbx6VP16-injected early gastrula with a condensation of pigmentation near injection sites. (C) Expression of Xbra in an uninjected early gastrula. (D) A Tbx6VP16-injected early gastrula showing a shift of Xbra expression domain to more anterior region. Arrowheads indicate the blastopore. (E) XMyf5 expression in an uninjected early gastrula. (F) Ectopic expression of XMyf5 in the dorsal region induced by Tbx6VP16. (G) Xwnt8 expression in an uninjected early gastrula. (H) Ectopic expression of Xwnt8 in a Tbx6VP16-injected early gastrula. (I) FGF8 expression in an uninjected early gastrula is localized to the entire marginal zone. (J) Dorsal injection of Tbx6VP16 shifted FGF8 expression to a more anterior region. (K) Sox17α expression is located in the yolk plug and latero-ventral blastoporal lip with lower level in the dorsal region. (L) Tbx6VP16 strongly induces ectopic Sox17α expression in the dorsal region. (M) Expression of the neural marker Sox3 in the dorsal ectoderm of an uninjected early gastrula. (N) Absence of Sox3 expression in the dorsal ectoderm following injection of Tbx6VP16. |
